Single-molecule localization microscopy (SMLM)

Our group is developing refined single-molecule imaging tools for super-resolution microscopy. We develop novel photoswitches, optical imaging schemes, software for image processing and data analysis, and apply these tools to study cellular life with a focus on membrane receptors.


  • Aug 2021: We have published our recent results on flat-field illumination and single-molecule photoswitching in ACS Photonics. Congrats Lucas & Paul!
  • Oct 2020: We have been successful in the EPSRC New Horizons scheme! In an interdisciplinary project with Gwyn Gould we aim at developing novel tools for membrane imaging at the nanoscale. More infos soon.
  • For recent SMLM software contributions see Software.

Recent publications:

  • Lucas Herdly, Paul Janin, Ralf Bauer, Sebastian van de Linde “Tunable Wide-Field Illumination and Single-Molecule Photoswitching with a Single MEMS Mirror” ACS Photonics 8, 2728–2736 (2021)
  • Mattia Marenda, Elena Lazarova, Sebastian van de Linde, Nick Gilbert, Davide Michieletto “Parameter-free molecular super-structures quantification in single-molecule localization microscopy” J. Cell Biol. 220, e202010003 (2021)
  • Christian Franke, Tomas Chum, Zuzana Kvicalova, Daniela Glatzová, Alvaro Rodriguez, Dominic Helmerich, Otakar Frank, Tomas Brdicka, Sebastian van de Linde, Marek Cebecauer “Unraveling nanotopography of cell surface receptors” bioRxiv (2020)